In [1]:
import pandas as pd
import numpy as np
import glob
import subprocess

datadir='/nagyvinyok/adat83/sotejedlik/ribli/dt40/bam_links_2/'

Start gfserver on jimgray83

In [2]:
%%writefile startblat.py
#!/usr/bin/python

import subprocess
#gfserver
gfserver_hostname,gfserver_port=" localhost "," 50000 "
gfServer="gfServer"

#reference genome
ref_fa="/home/ribli/input/index/gallus/Gallus_gallus.Galgal4.74.dna.toplevel.fa"
ref_2bit="/home/ribli/input/index/gallus/Gallus_gallus.Galgal4.74.dna.toplevel.2bit"

#starting gfserver for blasting
cmd0=gfServer + " start "+gfserver_hostname+gfserver_port
cmd0+=" -stepSize=5 -canStop -log=blatServer.log " + ref_2bit + " &"
subprocess.check_output(cmd0,shell=True)
Overwriting startblat.py
In [3]:
try:
    print subprocess.check_output(['sbatch','-C','jimgray85','--mem','1500','startblat.py' ])
except subprocess.CalledProcessError,e:
    print e.output
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Script to run crest

In [4]:
%%writefile dt40_chr_CREST.py
#!/usr/bin/python
import os
import subprocess
import sys

#tmpdir for CREST, because it leaves garbage
os.environ['TMPDIR']='/nagyvinyok/adat83/sotejedlik/ribli/temp/'

tumbam=sys.argv[1]
germbam=sys.argv[2]
chromos=sys.argv[3]

#gfServer
gfserver_hostname,gfserver_port=" localhost "," 50000 "

#exec path
CREST="/nagyvinyok/adat88/kozos/sotejedlik/usr/CREST/CREST.pl"
bam2html="/nagyvinyok/adat88/kozos/sotejedlik/usr/CREST/bam2html.pl"
extractSClip="/nagyvinyok/adat88/kozos/sotejedlik/usr/CREST/extractSClip.pl"
cap3="/nagyvinyok/adat88/kozos/sotejedlik/usr/CAP3/cap3"

#reference
ref_fa="/home/ribli/input/index/gallus/Gallus_gallus.Galgal4.74.dna.toplevel.fa"
ref_2bit="/home/ribli/input/index/gallus/Gallus_gallus.Galgal4.74.dna.toplevel.2bit"

#directory
output_dir="/nagyvinyok/adat83/sotejedlik/ribli/dt40/2016_08/sv/"

#run extractsclip, it finds soft clipping positions in bams
cmd_extr=extractSClip + " -i "+tumbam+" --ref_genome "+ref_fa
cmd_extr+=" -o "+output_dir + " -r "+chromos
print
print cmd_extr
subprocess.check_output(cmd_extr,shell=True)

#run CREST 
cmd_CREST= CREST + " -f "+output_dir+"/"+os.path.basename(tumbam)+"."+chromos+".cover "
cmd_CREST+=" -d "+tumbam+" -g " + germbam + " --ref_genome "+ref_fa+" -t "+ref_2bit 
cmd_CREST+="  -o "+output_dir+" --blatserver "+gfserver_hostname
cmd_CREST+=" --blatport "+gfserver_port+" --cap3 " + cap3 + " -r "+chromos 
cmd_CREST+=" -p " + os.path.basename(tumbam)+"."+chromos
print
print cmd_CREST
subprocess.check_output(cmd_CREST,shell=True)
Overwriting dt40_chr_CREST.py

Read the sample pairs

  • pairs columns was added by me
In [5]:
pairs=pd.read_excel('DT40 WGS Sample pairs.xlsx')

#ignore where pairs are na
pairs=pairs[~pd.isnull(pairs.Pair.values)]

pairs.head()
Out[5]:
Sample ID Genotype Treatment Corresponding starting clone Pair
0 DS161 pten starting clone NaN DS162
1 DS162 pten MMS DS161 DS161
2 DS163 pten MMS DS161 DS161
3 DS164 pten MMS DS161 DS161
4 DS165 pten cisplatin DS161 DS161

Get the bamfiles

In [6]:
bams,pair_bams=[],[]
for sample,pair in zip(pairs['Sample ID'],pairs['Pair']):
    bams.append(glob.glob(datadir+sample+'*.bam')[0])
    pair_bams.append(glob.glob(datadir+pair+'*.bam')[0])

Submit jobs

In [7]:
machine='jimgray85'
chromosomes=map(str,range(1,29)+[32,'W','Z'])

for sample,pair in zip(bams,pair_bams):
    for chromosome in chromosomes:  
        cmd_CREST= ['sbatch','-C',machine,'--mem','2000',
                    'dt40_chr_CREST.py',sample,pair,chromosome]
        try:
            print subprocess.check_output(cmd_CREST,stderr=subprocess.STDOUT),
        except subprocess.CalledProcessError,e:
            print e.output
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